ISO INTERNATIONAL STANDARD 11930 Secondedition 2019-01 Cosmetics Microbiology Evaluation of the antimicrobial protection of a cosmetic product Cosmetiques - Microbiologie Evaluation de la protection antimicrobienne d'un produit cosmétique Reference number IS0 11930:2019(E) osi @IS02019 IS0 11930:2019(E) COPYRIGHTPROTECTEDDOCUMENT @ IS0 2019 All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this publication may be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting on the internet or an intranet, without prior written permission. Permission can be requested from either IsO at the address below or Iso's member body in the country of the requester. ISO copyright office CP 401 : Ch. de Blandonnet 8 CH-1214 Vernier, Geneva Phone: +41 22 749 01 11 Fax: +41 22 749 09 47 Email: copyright@iso.org Website: www.iso.org Published in Switzerland ii @ IS0 2019 - All rights reserved IS0 11930:2019(E) Contents Page Foreword ..iv Introduction. .. 1 Scope. ..1 2 Normative references ..1 3 Terms and definitions ..1 4 Principle .2 5 Preservation efficacy test .3 5.1 General .3 5.2 Materials, apparatus, reagents and culture media .3 5.2.1 General 3 5.2.2 Materials .3 5.2.3 Diluents .3 5.2.4 Neutralizer 4 5.2.5 Culture media 5 5.3 Microbial strains .6 5.4 Preparation and enumeration of inocula 7 5.4.1 General .7 5.4.2 Preparation of bacterial and Candida albicans suspensions 7 5.4.3 Preparation of Aspergillus brasiliensis spore suspension .8 5.5 Demonstration of the neutralizer efficacy .9 5.5.1 Principle .9 5.5.2 Procedure. .9 5.5.3 Calculations 9 5.5.4 Interpretation of results and conclusion on neutralizer efficacy .10 5.6 Determination of the preservation efficacy of the formulation. ..10 5.6.1 Procedure .10 5.6.2 Counting of colonies.. .11 5.6.3 Calculations .11 5.7 Interpretation of test results and conclusions. .12 5.7.1 Criteria .12 5.7.2 General case (efficacy of the neutralizer is demonstrated for all strains) ..13 5.7.3 Case of formulations for which the efficacy of the neutralizer is not demonstratedforsomestrains ..13 5.8 Test report. ..13 Overall evaluation of the antimicrobial protection of the cosmetic product ..14 6.1 General .14 6.2 Case1 Preservation efficacy test has been performed on the formulation .14 6.3 Case 2 Preservation efficacy test has not been performed on the formulation ..15 Annex A (normative) Decision diagram ..16 Annex B (normative) Evaluation criteria for the preservation efficacy test ..17 Annex C (informative) Examples of neutralizers for the antimicrobial activity of preservatives and washing liquids ..18 Annex D (informative) Packaging characteristics ..20 Bibliography ..21 @ IS0 2019 - All rights reserved ii