ISO INTERNATIONAL STANDARD 12010 Secondedition 2019-03 Water quality - Determination of short-chain polychlorinated alkanes (SCCP) in water Method using gas chromatography-mass spectrometry (GC-MS) and negative-ion chemical ionization (NCI) Qualité de I'eau - Determination des alcanes polychlorés a chaine courte (ScCP) dans I'eau Méthode par chromatographie gazeuse-spectrométrie de masse (CG-SM) avec ionisation chimique négative (ICN) Reference number ISO 12010:2019(E) rso @ IS0 2019 IS0 12010:2019(E) COPYRIGHTPROTECTEDDOCUMENT @ IS0 2019 All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this publication may be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting on the internet or an intranet, without prior written permission. Permission can be requested from either IsO at the address below or Iso's member body in the country of the requester. ISO copyright office CP 401 : Ch. de Blandonnet 8 CH-1214 Vernier, Geneva Phone: +41 22 749 01 11 Fax: +41 22 749 09 47 Email: copyright@iso.org Website: www.iso.org Published in Switzerland ii @ IS0 2019 - All rights reserved IS0 12010:2019(E) Contents Page Foreword ..iv Introduction ..V 1 Scope. ..1 2 Normative references ..1 3 Terms and definitions ..1 4 Principle ..2 5 Interferences ..2 6 Reagents and standards. ..3 6.1 Solvents for extraction and preparation of stock solutions .3 6.2 ReferenceSCCPstocksolutions .3 6.3 Internal standard stock solutions from individual congeners .4 6.4 Calibration solutions .5 6.5 Extraction auxiliary and clean-up materials ..5 7 Apparatus. .6 8 Sampling and sample pretreatment .7 9 Procedure. .7 9.1 Extraction with liquid-liquid extraction .7 9.2 Extraction with higher content of suspended matter .7 9.3 Extractclean-up .8 9.4 Measurement and integration of the chromatogram. ..8 9.5 Calibration .9 9.5.1 General 9 9.5.2 Basiccalibration 10 9.5.3 Identification and quantification with mass fragment combinations .11 9.5.4 Calculation of the results ..11 9.5.5 Quality checks for internal standardization ..12 10 Expression of results ..12 11 Test report .12 Annex A (normative) Independent quality control check solutions ..13 Annex B (informative) Explanation of the calibration of the sum of SCCPs with multiple linearregression ..15 Annex C (informative) Typical GC-MS conditions ..21 Annex D (informative) Typical chromatograms of standard solutions and quality control check solutions 1 μg/ml... ..24 Annex E (informative) Presentation of goodness of fit ..31 Annex F (normative) Alternative clean-up with column chromatography ..32 Annex G (informative) Alternative clean-up with gel chromatography ..35 Annex H (informative) Chromatograms of real SPM samples ..36 Annex I (informative) Performance data ..40 Bibliography. ..42 @ IS0 2019 - All rights reserved ii