ISO INTERNATIONAL STANDARD 16014-2 Third edition 2019-05 Plastics Determination of average molecular weight and molecular weight distribution of polymers using size-exclusion chromatography - Part 2: Universal calibration method Plastiques-Determination de lamasse moleculairemoyenne etdeladistributiondes masses moleculairesdepolymeres par chromatographied'exclusionsterique Partie 2:Methode d'étalonnageuniverselle Referencenumber IS016014-2:2019(E) so @IS02019 IS016014-2:2019(E) COPYRIGHTPROTECTEDDOCUMENT IS02019 All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this publication may be reproduced or utilized otherwise in anyform or by any means, electronic or mechanical, including photocopying,or posting beloworIso'smemberbodyinthecountryoftherequester. ISO copyright office CP 401: Ch. de Blandonnet 8 CH-1214 Vernier, Geneva Phone:+41227490111 Fax:+4122749 0947 Email: copyright@iso.org Website: www.iso.org Published in Switzerland ii IS0 2019-All rights reserved IS016014-2:2019(E) Contents Page Foreword .iv 1 Scope. 2 Normativereferences .1 3 Terms anddefinitions ..1 4 Principleofuniversal calibrationmethod .1 5 Reagents 2 6 Apparatus. .2 7 Procedure .2 8 Data acquisitionand processing .3 9 Expressionofresults .3 9.1 Preparation ofuniversal calibration curve 3 9.2 Calculation of average molecular weight 3 9.3 Differentialmolecularweightdistributioncurve 4 9.4 Cumulativemolecularweightdistributioncurve 4 10 Precision 4 11 Testreport. 4 11.1 General 4 11.2 Apparatusandmeasurementparameters 4 11.3 Calibrationofthesystem .5 11.3.1Information on themolecular weight standards .5 11.3.2 Calibration curve. .5 11.4 Results .5 AnnexA(informative)Supplementaryinformation .6 Annex B (informative) Kand a in the Mark-Houwink-Sakurada equation .9 Bibliography .10 IS02019-All rights reserved ili