ISO INTERNATIONAL STANDARD 18593 Second edition 2018-06 Microbiology of the food chain - Horizontal methods for surface sampling Microbiologie de la chaine alimentaire Methodes horizontales pour les prélevements de surface Reference number ISO 18593:2018(E) so @IS02018 ut license from IHS Not for Resale, 07/10/2018 05:55:07 MDT IS0 18593:2018(E) COPYRIGHTPROTECTEDDOCUMENT @ IS0 2018 All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this publication may be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting on the internet or an intranet, without prior written permission. Permission can be requested from either IsO at the address below or Iso's member body in the country of the requester. ISO copyright office CP 401 : Ch. de Blandonnet 8 CH-1214 Vernier, Geneva Phone: +41 22 749 01 11 Fax: +41 22 749 09 47 Email: [email protected] Website: www.iso.org Published in Switzerland @ IS0 2018 - All rights reserved No reproduction or networking permited without license from IHS Not for Resale, 07/10/2018 05:55:07 MDT IS0 18593:2018(E) Contents Page Foreword ..iv Introduction. ..V 1 Scope. ..1 2 Normative references ..1 3 Terms and definitions 4 Principle ..2 5 Culture media and reagents ..2 6 Equipment and consumables ..3 7 Sampling procedure .3 7.1 General .3 7.2 Sampling location .3 7.3 Sampling area. .4 7.4 Time of sampling and frequency 4 7.5 Sampling techniques.. 4 7.5.1 General .4 7.5.2 Contact plate method 5 7.5.3 Stick swab method .5 7.5.4 Sponge/cloth method. 5 8 Storage and transport .6 8.1 Contactplate .6 8.2 Stick swab, sponge/cloth .6 Microbiological analysis of samples .6 9.1 Contact plate method 9.2 Stick swab/cloth/sponge method .. 6 10 Expression of results and calculation .7 1 10.1 General 10.2 Contact plate method 10.3 Method using stick swab, cloth or sponge ..7 11 Test report ..7 Annex A (informative) Neutralizers .9 Bibliography 11 pyrntnematonal rights reerved ii without license from IHS Not for Resale, 07/10/2018 05:55:07 MDT