ISO INTERNATIONAL STANDARD 21285 First edition 2019-04 Soil quality Inhibition of reproduction of the soil mite (Hypoaspis aculeifer) by soil contaminants Qualité du sol - Inhibition de la reproduction de I'acarien prédateur (Hypoaspis aculeifer) par des contaminants du sol Reference number ISO 21285:2019(E) osi @IS02019 IS0 21285:2019(E) COPYRIGHTPROTECTEDDOCUMENT @ IS0 2019 All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this publication may be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting on the internet or an intranet, without prior written permission. Permission can be requested from either IsO at the address below or Iso's member body in the country of the requester. ISO copyright office CP 401 : Ch. de Blandonnet 8 CH-1214 Vernier, Geneva Phone: +41 22 749 01 11 Fax: +41 22 749 09 47 Email: copyright@iso.org Website: www.iso.org Published in Switzerland ii @ IS0 2019 - All rights reserved IS0 21285:2019(E) Contents Page Foreword ..iv Introduction. .. 1 Scope. ..1 2 Normative references 3 Terms and definitions ..2 4 Principle ..3 5 Reagents and material .4 5.1 Biological material .4 5.2 Test mixtures ..4 5.3 Reference substance. ..5 6 Apparatus. .6 7 Procedure .6 7.1 Experimental design .6 7.1.1 General. .6 7.1.2 Range-finding test (preliminary test) .7 7.1.3 Definitivetest 7 7.1.4 Limittest .7 7.2 Preparation of test mixtures .8 7.2.1 Testing contaminated soil .8 7.2.2 Testing substances added to the test substrate .8 7.2.3 Preparation of control containers .9 7.3 Addition of the biological material .9 7.4 Test conditions and measurements .9 7.5 Feeding of the mites. .9 7.6 Determination of surviving predatory mites .10 8 Calculation and expression of results .10 8.1 Calculation .10 8.2 Expression of results. ..10 9 Validity of the test .10 10 Statistical analysis .11 10.1 General 11 10.2 Single-concentration tests. .11 10.3 Multi-concentration tests. .11 10.3.1 Range-finding test 11 10.3.2 Definitive test. .11 11 Test report. ..12 Annex A (informative) Techniques for rearing and breeding of predatory mites ..14 Annex B (normative) Determination of water-holding capacity ..16 Annex C (informative) Guidance on adjustment of pH of artificial soil ..17 Annex D (informative) Extraction and counting of predatory mites ..18 Annex E (informative) Basic information on the biology of Hypoaspis (Geolaelaps) aculeifer...19 Bibliography .20 @ IS0 2019 - All rights reserved ii