ISO INTERNATIONAL STANDARD 21315 First edition 2018-12 Traditional Chinese medicine Ganoderma lucidum fruiting body Medecine traditionnelle chinoise-Organe fructiferede Ganoderma lucidum Reference number IS021315:2018(E) @IS02018 IS0 21315:2018(E) COPYRIGHTPROTECTEDDOCUMENT @ ISO 2018 All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this publication may be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting on the internet or an intranet, without prior written permission. Permission can be requested from either IsO at the address belowor Iso'smemberbody in the country ofthe requester. ISO copyright office CP 401 : Ch. de Blandonnet 8 CH-1214 Vernier, Geneva Phone:+41227490111 Fax:+412274909 47 Email: [email protected] Website:www.iso.org Published in Switzerland ii @ IS0 2018 - All rights reserved IS021315:2018(E) Contents Page Foreword .iv Introduction .V 1 Scope. 1 2 Normativereferences 1 3 Terms and definitions 1 4 Descriptions 5 Requirements 2 5.1 General characteristics 2 5.2 Ganoderma lucidum fruiting body 3 5.2.1 Morphologicalfeaturesoffruitingbody 3 5.2.2 Thin-layer chromatogram (TLC) identification 3 5.2.3 Moisture 3 5.2.4 Total ash 3 5.2.5 Water-soluble extractives 3 5.2.6 Marker compounds. 3 5.2.7 Heavymetals 3 5.2.8 Pesticideresidues 6 Sampling. .4 7 Test methods 4 7.1 Macroscopic identification 4 7.2 TLCidentification 4 7.3 Determination ofmoisturecontent 4 7.4 Determination of total ash 4 7.5 Determinationofwater-solubleextractives 4 7.6 Determination of marker compounds 4 7.7 Determination of heavy metals 4 7.8 Determination of pesticide residues 4 8 Testreport .4 9 Packaging,storageand transportation .5 10 Marking and labelling .5 AnnexA (informative)TLCidentification .6 AnnexB(informative)Determinationofwater-solubleextractives .8 AnnexC (informative)Determinationofpolysaccharides .9 Annex D (informative) Determination of ganoderic acid A 11 Annex E (informative) Reference values of national and regional limits ofmoisture,total ash, water-soluble extractives, polysaccharides, ganoderic acid Aand heavy metals in Ganoderma lucidum fruiting body 14 Bibliography 15 @ IS0 2018 - All rights reserved ii