ISO INTERNATIONAL STANDARD 21370 First edition 2019-02 Traditional Chinese medicine - Dendrobium officinale stem Médecine traditionnelle chinoise - Tige de Dendrobium officinale Reference number IS0 21370:2019(E) osi @IS02019 IS0 21370:2019(E) COPYRIGHTPROTECTEDDOCUMENT @ IS0 2019 All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this publication may be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting on the internet or an intranet, without prior written permission. Permission can be requested from either IsO at the address below or Iso's member body in the country of the requester. ISO copyright office CP 401 : Ch. de Blandonnet 8 CH-1214 Vernier, Geneva Phone: +41 22 749 01 11 Fax: +41 22 749 09 47 Email: [email protected] Website: www.iso.org Published in Switzerland ii @ IS0 2019 - All rights reserved IS0 21370:2019(E) Contents Page Foreword ..iv Introduction. ..V 1 Scope. ..1 2 Normative references ..1 3 Terms and definitions ..1 4 Descriptions .2 5 Requirements .2 5.1 General characteristics .2 5.2 Dendrobium officinale stem 3 5.2.1 Morphological features 3 5.2.2 Thin-layer chromatogram (TLC) identification .3 5.2.3 Moisture 3 5.2.4 Total ash .3 5.2.5 Ethanol-soluble extractives 3 5.2.6 Marker compounds. 3 5.2.7 Heavymetals .3 5.2.8 Pesticide residues 4 6 Sampling. :4 7 Test methods. .4 7.1 Macroscopicidentification .4 7.2 TLC identification ..4 7.3 Determination of moisture content .4 7.4 Determination of total ash 4 7.5 Determination of ethanol-soluble extractives .4 7.6 Determination of marker compounds .4 7.7 Determination of heavy metals 7.8 Determination of pesticide residues .4 8 Test report ..4 9 Packaging, storage and transportation .5 10 Marking and labelling ..5 Annex A (informative) TLC identification ..6 Annex B (informative) Determination of ethanol-soluble extractives ..8 Annex C (informative) Determination of polysaccharides 9 Annex D (informative) Determination of mannose and peak area ratio of mannose to glucose....11 Annex E (informative) Reference values of national and regional limits of moisture, total ash, ethanoi-soiubie extractives, poiysaccharides, mannose and peak area ratio of mannose to glucose in Dendrobium officinale stem 14 Bibliography .15 @ IS0 2019 - All rights reserved ii