ISO/TR TECHNICAL REPORT 20489 First edition 2018-12 Nanotechnologies Sample preparation for the characterization of metal and metal-oxide nano-objects in water samples Nanotechnologies - Préparation des échantillons pour la caracterisation de nano-objets métalliques et d'oxydes métalliques dansleséchantillonsd'eau Reference number IS0/TR 20489:2018(E) rso @IS02018 IS0/TR 20489:2018(E) COPYRIGHTPROTECTEDDOCUMENT @ IS0 2018 All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this publication may be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting on the internet or an intranet, without prior written permission. Permission can be requested from either IsO at the address below or Iso's member body in the country of the requester. ISO copyright office CP 401 : Ch. de Blandonnet 8 CH-1214 Vernier, Geneva Phone: +41 22 749 01 11 Fax: +41 22 749 09 47 Email: copyright@iso.org Website: www.iso.org Published in Switzerland ii @ IS0 2018 - All rights reserved IS0/TR 20489:2018(E) Contents Page Foreword ..iv Introduction. ..V 1 Scope. ..1 2 Normative references 3 Terms and definitions 4 Symbols and abbreviated terms ..2 5 Types of metal and metal oxide-based manufactured nano-objects ..2 6 Types of water matrices of interest ..2 7 Sample collection and storage .3 7.1 .3 General. 7.2 Containers for sample collection and storage .3 8 Sample pre-treatment .3 8.1 Introduction ..3 8.2 Sedimentation and centrifugation 4 8.2.1 Sedimentation 4 8.2.2 Centrifugation .4 8.2.3 Stepwise sedimentation and centrifugation ..4 8.2.4 Factors affecting centrifugation and sedimentation ..4 8.2.5 Advantages and limitations of centrifugation .5 8.3 Filtration .5 9 Size fractionation techniques. .5 9.1 Introduction 5 9.2 Field-flow fractionation (FFF) .6 9.2.1 Introduction .6 9.2.2 Advantages and limitations .. 6 9.3 Ultrafiltration .7 9.4 Size exclusion chromatography .7 Annex A (informative) Relevant nano-object characterization techniques ..8 Bibliography 9 @ IS0 2018 - All rights reserved ii