ISO INTERNATIONAL STANDARD 12228-1 Firstedition 2014-07-15 Determination of individual and total sterols contents Gas chromatographic method - Part 1: Animal and vegetable fats and oils Détermination dela teneur en stérols individuels et totaux- Méthodeparchromatographieenphasegazeuse- Partie 1: Corps gras d'originesanimale et vegetale Reference number IS012228-1:2014(E) LSO @IS02014 Not for Resale IS012228-1:2014(E) COPYRIGHTPROTECTEDDOCUMENT @ISO2014 All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting on the internet or an intranet, without prior written permission. Permission can be requested from either ISO at the address below or ISO's member body in the country of the requester. ISO copyright office Case postale 56.CH-1211 Geneva 20 Tel. + 4122 749 0111 Fax +41 22 749 09 47 E-mail copyright@iso.org Web www.iso.org Published in Switzerland @ IS0 2014- All rights reserved No reproduction or networking permited without license from IHS Not for Resale IS012228-1:2014(E) Contents Page Foreword ..iv 1 Scope ..1 2 Normative references 3 Terms and definitions 4 Principle 5 Reagents. ..2 6 Apparatus .3 7 Sample. .3 7.1 Sampling .3 7.2 Preparation of the test sample 8 Procedure. ..4 8.1 Preparation ofthealuminium oxidecolumn .4 8.2 Testportion 4 8.3 Extraction of theunsaponifiablematter .4 8.4 Thin-layer chromatography.. .4 8.5 Isolation of the sterols 4 8.6 Preparation of sterol trimethylsilyl ethers .5 8.7 Gas chromatography .5 6 Expression of results .5 9.1 Identification of sterols .5 9.2 Composition of sterols. .5 9.3 Determination ofthetotal sterol content .6 10 Precision .7 10.1 Interlaboratory test. 7 10.2 Repeatability limit, r .7 10.3 Reproducibility limit, R 7 11 Test report ..7 AnnexA(informative)Figures .8 Annex B (informative) Interlaboratory trial .15 Bibliography .23 iii IHS underI thout license from IHS Not for Resale