ISO INTERNATIONAL STANDARD 12228-2 First edition 2014-10-01 Determination of individual and total sterols contents Gas chromatographic method - Part 2: Olive oils and olive pomace oils Détermination de la teneur en stérols individuels et totaux - Méthode par chromatographie en phase gazeuse - Partie 2: Huile d'olive et huile de grignons d'olive Reference number IS0 12228-2:2014(E) ISO @IS02014 mitted without license from IHS Not for Resale IS0 12228-2:2014(E) COPYRIGHT PROTECTED DOCUMENT @ISO2014 All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized otherwise in any form written permission. Permission can be requested from either ISO at the address below or ISO's member body in the country of the requester. ISO copyright office Case postale 56 : CH-1211 Geneva 20 Tel. + 41 22 749 01 11 Fax +41 22 749 09 47 E-mail [email protected] Web www.iso.org Published in Switzerland @ IS0 2014 - All rights reserved No reproduction or networking permited without license from IHS Not for Resale IS0 12228-2:2014(E) Contents Page Foreword ..iv 1 Scope .1 2 Normative references ..1 3 Terms and definitions 4 Principle 5 Reagents. ..2 6 Apparatus .3 7 Sample. ..4 7.1 Sampling ..4 7.2 Preparation of the test sample .4 8 Procedure 8.1 Testportion .4 8.2 Preparation of unsaponifiable matter. 4 8.3 Separation of the sterol and triterpene dialcohols (erythrodiol, uvaol) fractions by TLC ... 5 8.4 Preparationofthetrimethylsilylethers. .6 8.5 Gas chromatographic analysis. 6 6 Expression of results. .7 9.1 Quantitative evaluation 7 9.2 Determination of the total sterol content .8 9.3 Composition of sterols. .8 9.4 Composition of triterpene dialcohols. 10 Precision .9 10.1 Interlaboratory test. .9 10.2 Repeatability limit, r ..9 10.3 Reproducibility limit, R ..9 11 Test report .9 Annex A (informative) Figures. ..10 Annex B (informative) Interlaborative trial ..13 Bibliography ..16 iii without license from IHS Not for Resale