ISO INTERNATIONAL STANDARD 12966-1 First edition 2014-12-01 Animal and vegetable fats and oils - Gas chromatography of fatty acid methyl esters Part 1: Guidelines on modern gas chromatography of fatty acid methyl esters Corps gras d'origines animale et vegétale - Chromatographie en phase gazeuse des esters méthyliques d'acides gras - Partie 1: Lignes directrices relatives a la chromatographie en phase gazeuse moderne des esters méthyliques d'acides gras Reference number ISO 12966-1:2014(E) SO International Organization for Standardization @ IS0 2014 dardization5956617 ed without license from IHS IS0 12966-1:2014(E) COPYRIGHTPROTECTEDDOCUMENT @ IS0 2014 All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting on the internet or an intranet, without prior written permission. Permission can be requested from either ISO at the address below or ISO's member body in the country of the requester. ISOcopyrightoffice Case postale 56:CH-1211 Geneva 20 Tel. + 41 22 749 01 11 Fax + 41 22 749 09 47 E-mail copyright@iso.org Web www.iso.org Published in Switzerland Prganizationfor Standardization Not for Resale, 2015/3/20 05:25:14 networking permitted without license from IHS IS0 12966-1:2014(E) Contents Page Foreword ..iv Introduction. ..V 1 Scope. ..1 2 Normative references ..1 3 Principle 4 Preparation of FAME 5 Columns. ..3 6 GLCofFAMEs 7 Evaluation of the chromatograms ..4 7.1 Peak area and area per cent. 7.2 Evaluation by means of an internal standard or correction factors .4 8 Test report ..5 Bibliography 6 iii vided by IHS underI se with ISt ed without license from IHS