ISO INTERNATIONAL STANDARD 12966-3 Secondedition 2016-05-15 Animal and vegetable fats and oils - Gas chromatography of fatty acid methyl esters Part 3: Preparation of methyl esters using trimethylsulfonium hydroxide (TMSH) Corps gras d'origines animale et végétale Chromatographie en phase gazeuse des esters méthyliques d'acides gras Partie 3: Preparation des esters meéthyliques a I'aide d'hydroxyde de triméthylsulfonium (TMSH) Reference number ISO 12966-3:2016(E) ISO International Organization for Standardization @ IS0 2016 STANDARDIZATION C15956617 I without license from IHS IS0 12966-3:2016(E) COPYRIGHTPROTECTEDDOCUMENT IS0 2016, Published in Switzerland All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized otherwise in any form written permission. Permission can be requested from either ISO at the address below or ISO's member body in the country of the requester. ISO copyright office Ch. de Blandonnet 8 . CP 401 CH-1214 Vernier, Geneva, Switzerland Tel. +41 22 749 01 11 Fax +41 22 749 09 47 copyright@iso.org www.iso.org Internatinair PrganizationforStandardization ensee-ZHEJIANG INSTOFSTANDARDIZAoISQ6- All rights reserved etworking permitted without license from IHS IS0 12966-3:2016(E) Contents Page Foreword ..iv 1 Scope .1 2 Normative references 3 Principle. 4 Reagents 5 Apparatus 6 Sampling. 7 Preparation of the test sample ..2 8 Procedure. ..2 9 Test report. ..3 Bibliography .4 Inteatinal oganzation @st(Sr201.6 - All rights reserved iii See=ZHEJIANG INST OF STANDARDIZATION C1 5956617 vided by IHS under lic se with ISi ed without license from IHS