ISO INTERNATIONAL STANDARD 12966-4 First edition 2015-06-01 Animal and vegetable fats and oils - Gas chromatography of fatty acid methyl esters Part 4: Determination by capillary gas chromatography Corps gras d'origines animale et vegétale - Chromatographie en phase gazeuse des esters méthyliques d'acides gras Partie 4: Détermination par chromatographie capillaire en phase gazeuse Reference number ISO 12966-4:2015(E) ISO International Organization for Standardization @ IS0 2015 nstitute of Standardization 5956617 ed without license from IHS IS0 12966-4:2015(E) COPYRIGHTPROTECTEDDOCUMENT IS02015,PublishedinSwitzerland All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting on the internet or an intranet, without prior written permission. Permission can be requested from either ISO at the address below or ISO's member body in the country of the requester. ISO copyright office Ch. de Blandonnet 8 . CP 401 CH-1214 Vernier, Geneva, Switzerland Tel. +41 22 749 01 11 Fax+412274909 47 copyright@iso.org www.iso.org Intenationai br PrganizationforStandardization Not for Resale, 2015/8/7 05:29:3 etworking permitted without license from IHS IS0 12966-4:2015(E) Contents Page Foreword ..iv 1 Scope 2 Normative references ..1 3 Principle 4 Reagents and materials ..1 4.1 Reference fatty acid methyl esters (FAMEs) .2 4.2 Internal standards. ..2 5 Apparatus ..3 6 Sampling. ..4 7 Preparation of test sample .4 8 Preparation of methyl esters from fats, oils, and fatty acids ..4 9 Procedure. ..4 9.1 General ..4 9.2 GCconditions .4 9.3 Performance check .5 10 Calculations. .5 10.1 Qualitative analysis and peak identification .5 10.2 Quantitative analysis .5 10.2.1 Calculation ofthe composition offattyacid methyl esters ..5 10.2.2 Calculation of the composition of fatty acid methyl esters using correction factors .6 10.2.3 Calculation of the composition of fatty acid methyl esters using an internalstandard .6 11 Precision .7 11.1 Results of interlaboratory test. 7 11.2 Repeatability .7 11.3 Reproducibility .7 12 Test report 7 Annex A (informative) Theoretical flame ionization detector correction factor (TcF) for fatty acid methyl esters (FAMEs) 9 Annex B (informative) Examples of chromatograms ..10 Annex C (informative) Comparison of FAME composition with two different GC columns. ..12 Annex D (informative) Results of an interlaboratory trial ..14 Bibliography .20 iii nstitute of Standardization 5956617 thout license from IHS