FINAL INTERNATIONAL ISO/FDIS DRAFT STANDARD 14189 ISO/TC147/SC4 Water quality - Enumeration of Secretariat: DIN Clostridium perfringens Method Voting begins on: 2013-07-29 using membrane filtration Voting terminates on: Qualité de I'eau - Dénombrement de Clostridium perfringens - 2013-09-29 Méthode de filtration sur membrane SUBMIT, WITH THEIR COMMENTS, NOTIFICATION OF ANY RELEVANT PATENT RIGHTS OF WHICH THEY ARE AWARE AND TO PROVIDE SUPPORTING DOCUMENTATION INADDITIONTOTHEIREVALUATIONAS Reference number BEING ACCEPTABLE FOR INDUSTRIAL, TECHNO- LOGICAL, COMMERCIAL AND USER PURPOSES, IS0/FDIS14189:2013(E) DRAFT INTERNATIONAL STANDARDS MAY ON ISO OCCASION HAVE TO BE CONSIDERED IN THE DARDS TO WHICHREFERENCE MAYBE MADEIN @IS02013 by IHS underI license from IHS Not for Resale IS0/FDIS 14189:2013(E) COPYRIGHTPROTECTEDDOCUMENT @ IS0 2013 All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized otherwise in any form written permission. Permission can be requested from either ISO at the address below or ISO's member body in the country of the requester. ISO copyright office Case postale 56:CH-1211 Geneva 20 Tel. + 41 22 749 01 11 Fax +4122 749 09 47 E-mail copyright@iso.org Web www.iso.org Published in Switzerland @ IS0 2013 - All rights reserved No reproductic networking permited without license from IHS Not for Resale IS0/FDIS 14189:2013(E) Contents Page Foreword ..iv Introduction. ..V 1 Scope. ..1 2 Normative references ..1 3 Terms and definitions 4 Principle ..2 5 Apparatus and glassware .2 6 Culture media and reagents .2 6.1 Basic materials .2 6.2 Culture media. .3 7 Sampling. ..3 8 Procedure .3 8.1 Transport and storage of the sample .3 8.2 Heat pre-treatmentto select spores. .3 8.3 Sample dilution. .3 8.4 Filtration. .3 8.5 Incubationand examination .4 8.6 Confirmation of Clostridium perfringens ..4 9 Expression of results ..5 10 Test report. 11 Quality assurance .5 Annex A (normative) Composition and preparation of culture media and reagents ..7 Annex B (informative) Performancedata .9 Bibliography .12 iii se with ISt thout license from IHS Not for Resale