ISO INTERNATIONAL STANDARD 17244 First edition 2015-09-15 Water quality - Determination of the toxicity of water samples on the embryo-larval development of Japanese oyster (Crassostrea gigas) and mussei (Mytilus edulis or Mytilus galloprovincialis) Qualité de I'eau - Détermination de la toxicité d'eéchantillons aqueux sur le développement embryo-larvaire de I'huitre creuse (Crassostrea gigas) et de la moule (Mytilus edulis ou Mytilus galloprovincialis) Reference number ISO 17244:2015(E) ISO International Organization for Standardization @ IS0 2015 rdization 5956617 without license from IHS IS0 17244:2015(E) COPYRIGHTPROTECTEDDOCUMENT IS02015,PublishedinSwitzerland All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting on the internet or an intranet, without prior written permission. Permission can be requested from either ISO at the address below or ISO's member body in the country of the requester. ISO copyright office Ch. de Blandonnet 8 . CP 401 CH-1214 Vernier, Geneva, Switzerland Tel. +41 22 749 01 11 Fax +41 22 749 09 47 copyright@iso.org www.iso.org Intenationaibr Prganization for Standardization icene-zZhejag nsite f StandardiationSsJS9 2015 - All rights reserved etworking permitted without license from IHS IS0 17244:2015(E) Contents Page Foreword ..iv Introduction. ..V 1 Scope. ..1 2 Normative references ..1 3 Terms and definitions 4 Principle ..2 5 Test organisms and seawater .2 5.1 Spawning stock or mature bivalves. .2 5.2 Referenceseawater .3 5.2.1 Natural seawater .3 5.2.2 Synthetic seawater 3 5.2.3 Hypersaline brine (HSB) 4 6 Equipment .4 7 Reference substance. .5 8 Test procedure .5 8.1 Collection, preparation, and preservation of aqueous samples 5 8.2 Preparation of test samples. .5 8.2.1 Chemicals.. .5 8.2.2 Aqueous samples .5 8.3 Selection of concentration/dilution range .6 8.4 Collecting gametes. .6 8.4.1 General. .6 8.4.2 Thermal stimulation. .6 8.4.3 Stripping the gametes .8 8.5 Measurement of egg density .8 8.6 Fertilization and inoculation of fertilized eggs 8 8.7 Incubation .9 8.8 Observation .9 8.9 Analytical measurements 10 9 Expression of results ..10 10 Validity criteria ..12 11 Test report. .12 Annex A (informative) Overview of the test applied to the Japanese oyster Crassostrea gigas. Annex B (informative) Performance data ..14 Bibliography .22 iii vided by IHS under lic without license from IHS