ISO INTERNATIONAL STANDARD 17604 Secondedition 2015-08-15 Microbiology of the food chain Carcass sampling for microbiological analysis Microbiologie dela chainealimentaire-Prelevement d'échantillons sur des carcasses en vue de leur analyse microbiologique Reference number ISO17604:2015(E) ISO Intermational Organization for Standardization @IS02015 itted without license from IHS IS017604:2015(E) COPYRIGHTPROTECTEDDOCUMENT IS02015,Published in Switzerland All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting on the internet or an intranet, without prior written permission. Permission can be requested from either ISO at the address below or ISO's member body in the country of the requester. ISOcopyrightoffice Ch. de Blandonnet 8. CP 401 CH-1214 Vernier, Geneva, Switzerland Tel. +41 22 749 01 11 Fax +41 22 749 09 47 [email protected] www.iso.org Intematinaibr PrganizationfoStandardization networking permited without license from IHS NotforResale, 2015/11/11 08:21:49 IS017604:2015(E) Contents Page Foreword ..iv Introduction.. ... 1 Scope.. 2 Normative references 3 Terms and definitions 4 General principles ..2 5 Sampling plans. .2 6 Samplingpoints on theproduction line 7 Sampling siteson carcasses .3 7.1 Large mammals. 7.2 Poultry, small mammals, and other small animals. 4 7.3 Game. .4 8 Sampling techniques ..4 8.1 General 4 8.2 Excision methods 4 8.2.1 Cork borer method .4 8.2.2 Template excision method .5 8.2.3 Skin sampling. 8.3 Swabbing methods. 6 8.3.1 Wetanddrystickswabmethod .6 8.3.2 Sponge, tampon swab, and small cloth method .6 8.4 Carcass rinsing method .7 8.4.1 Reagent. .7 8.4.2 Equipment. .7 8.4.3 Collection of samples .7 9 Storage and transport of samples. ..8 10 Microbiological examination of samples ..8 10.1 General .8 10.2 Initialpreparation ofsamples. ..8 10.2.1Swabbing methods. .8 10.2.2 Excised samples.. .8 10.2.3 Carcassrinsesamples. .8 10.3 Enumeration and detection of microorganisms Annex A (informative) Sampling sites .9 Bibliography ..13 iii see=Zhejiang Institute of Standardization 5956617 ithout license from IHS