ISO INTERNATIONAL STANDARD 18187 First edition 2016-05-01 Soil quality Contact test for solid samples using the dehydrogenase activity of Arthrobacter globiformis Qualité du sol-Essai contact pour échantillons solides utilisant I'activite deshydrogenase de Arthrobacter globiformis Reference number ISO 18187:2016(E) ISO International Organization for Standardization =ZHEJIANG INST OF STANDARDIZATION C1 5956617 @IS02016 vided by IHS under ted without license from IHS IS0 18187:2016(E) COPYRIGHTPROTECTEDDOCUMENT IS0 2016, Published in Switzerland All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized otherwise in any form written permission. Permission can be requested from either ISO at the address below or ISO's member body in the country of the requester. ISOcopyrightoffice Ch. de Blandonnet 8 . CP 401 CH-1214 Vernier, Geneva, Switzerland Tel. +41 22 749 01 11 Fax +41 22 749 09 47 copyright@iso.org www.iso.org Internatinair PrganizationforStandardization ensee-ZHEJIANG INST.OF STANDARDIzARoISQ16- All rights reserved Not for Resale, 2016/7/20 03:26:45 etworking permitted without license from IHS IS0 18187:2016(E) Contents Page Foreword ..iv Introduction. ..V 1 Scope. ..1 2 Normative references ..1 3 Terms and definitions 4 Principle ..4 5 Reagents and material ..4 5.1 Test organisms .4 5.2 Control substrates. 5.2.1 General .4 5.2.2 Control for soils. ..4 5.2.3 Control for waste material ..5 5.3 Test substrates .5 5.4 Chemicals. .6 6 Apparatus 8 7 Procedure .9 7.1 Preparation of dilutions 7.2 Reference substance and positive control preparation .9 7.3 Contact test procedure ..9 7.3.1 General. ..9 7.3.2 Aeration .10 7.3.3 Deactivation .10 7.3.4 Preparation of the inoculum .11 7.3.5 Incubationandfluorescencemeasurement .11 7.4 Interferences. .11 8 Calculation and expression of results .12 8.1 Calculation .12 8.1.1 Relative fluorescence ..12 8.1.2 Determining the percentage of inhibition ..12 8.2 Expression of results. .12 9 Validity of the test ..13 10 Statistical analysis ..13 11 Test report. ..14 Annex A (informative) Results on the ring test .15 Annex B (informative) Preparation of test organisms ..21 Annex C (informative) Testing chemical substances. .23 Bibliography .25 iii See=ZHEJIANG INST OF STANDARDIZATION C1 5956617 ed withoutlicense from IHS