ISO INTERNATIONAL STANDARD 18465 First edition 2017-01 Microbiology of the food chain Quantitative determination of emetic toxin (cereulide) using LC-MS/MS Microbiologie de la chaine alimentaire - Deétermination quantitative de la toxine émétique (cereulide) par CL-SM/SM Reference number IS0 18465:2017(E) International Organization for Standardization @ IS0 2017 e=ZHEJIANG INST OF STANDARDIZATION C1 5956617 mitted without license from IHS IS0 18465:2017(E) COPYRIGHT PROTECTEDDOCUMENT IS02017,PublishedinSwitzerland All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting on the internet or an intranet, without prior written permission. Permission can be requested from either ISO at the address below or ISO's member body in the country of the requester. ISO copyrightoffice Ch. de Blandonnet 8 . CP 401 CH-1214 Vernier, Geneva, Switzerland Tel. +41 22 749 01 11 Fax +41 22 749 09 47 copyright@iso.org www.iso.org Licensee-ZHEJIANG INST OF STANDARDIzRoISQ 7 - All rights reserved ded by IHS unde Not for Resale, 2017/3/28 02:51:21 networking permited without license from IHS IS0 18465:2017(E) Contents Page Foreword ..iv Introduction. ..V 1 Scope. ..1 2 Normative references 3 Terms and definitions 4 General principle ..1 5 Reagents ..1 6 Apparatus and equipment ..3 7 Procedure .4 7.1 Sample preparation .4 7.2 Standard preparation 4 7.3 LC-MS analysis. .5 7.3.1 LC conditions 5 7.3.2 MS conditions and tuning parameters 5 7.3.3 Transitions (multiple reaction monitoring, MRM) .6 8 Calculation .6 9 Quality controls ..7 10 Precision ..8 10.1 General. ..8 10.2 Repeatability 10.3 Reproducibility ..9 Annex A (informative) Results of the interlaboratory study ..10 Annex B (informative) Possible transitions of cereulide in Ms[3] ..12 Bibliography. ..14 iii icensee=ZHEJIANG INST OF STANDARDIZATION C1 5956617 without license from IHS