ISO INTERNATIONAL STANDARD 18862 First edition 2016-07-15 Coffee and coffee products - Determination of acrylamide Methods using HPLC-MS/MS and GC- MS after derivatization Cafe et de ses dérivés -Dosage de I'acrylamide Méthodes utilisant CLHP-MS/MS et CG-MS apres dérivation Reference number ISO 18862:2016(E) ISO International Organization for Standardization ZHEJIANG INSTOFSTANDARDIZATION C15956617 @IS02016 vided by IHS underI ted without license from IHS IS0 18862:2016(E) COPYRIGHTPROTECTEDDOCUMENT IS02016,PublishedinSwitzerland All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting on the internet or an intranet, without prior written permission. Permission can be requested from either ISO at the address below or ISO's member body in the country of the requester. ISO copyright office Ch. de Blandonnet 8 . CP 401 CH-1214 Vernier, Geneva, Switzerland Tel.+41 22 749 01 11 Fax +41227490947 copyright@iso.org www.iso.org Intenationai br PrganizationforStandardization icensee-ZHEJIANG INST OF STANDARDIZoISQ s.6 - All rights reserved I by IHS unde Not for Resale, 2016/9/22 06:22:13 etworking permitted without license from IHS IS0 18862:2016(E) Contents Page Foreword ..iv Introduction. ..V 1 Scope. ..1 2 Normative references 3 Terms and definitions 4 Principle .1 5 Reagents. 6 Apparatus. ..3 7 Sampling. ..4 8 Procedure. .4 8.1 General .4 8.2 Preparation of the sample extract 4 8.3 Clean-up of the extracts .5 8.3.1 Carrez precipitation.. .5 8.3.2 Solid phase extraction .5 8.4 HPLC-MS/MS measurement. .5 8.4.1 High-performance liquid chromatography (HPLC) ..5 8.4.2 Identification and quantification by mass spectrometry (HPLC-MS/MS) ..6 8.5 Measurement with GC-MS .6 8.5.1 Derivatization and sample preparation for gas chromatography ..6 8.5.2 Gas chromatography. 7 8.5.3 Identification and quantification bymass spectrometry .7 9 Calibration 9.1 General advice .7 9.2 Determination of linearity and definition of the working range ..7 9.3 Calibration with internal standard solution .7 9.4 Determination of the laboratory specific recovery ..8 10 Evaluation ..8 10.1 Criteria for identification .8 10.2 Calculation and final results ..8 11 Precision data .9 11.1 General .9 11.2 Repeatability .9 11.3 Reproducibility 11.4 Recovery .9 12 Measurement uncertainty .9 13 Test report. .10 Annex A (informative) Performance characteristics .11 Annex B (informative) Examples of absorber materials .12 Annex C (informative) Examples of columns and analysis conditions ..13 Bibliography .19 iii ee=ZHEJIANG INSTOF STANDARDIZATION C15956617 withoutlicense from IHS