ISO INTERNATIONAL STANDARD 19820 First edition 2016-03-01 Water quality Determination of the acute toxicity to the marine rotifer Brachionus plicatilis Qualite de I'eau - Détermination de la toxicité aigue envers le rotifere marin Brachionus plicatilis Reference number IS0 19820:2016(E) ISO International Organization for Standardization see=ZHEJIANG INST OF STANDARDIZATION C1 5956617 @IS02016 No reproduction or networking permitted without license from IHS IS0 19820:2016(E) COPYRIGHT PROTECTED DOCUMENT IS0 2016, Published in Switzerland All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized otherwise in any form written permission. Permission can be requested from either ISO at the address below or ISO's member body in the country of the requester. ISOcopyrightoffice Ch. de Blandonnet 8 · CP 401 CH-1214 Vernier, Geneva, Switzerland Tel. +41 22 749 01 11 Fax +41 22 749 09 47 copyright@iso.org www.iso.org Internatinir DrganizationforStandardization Licensee-ZHEJIANG INST OF STANDARDIZoISQ6 -All rights reserved Not for Resale, 2016/4/26 02:58:10 networking permited withoutlicense from IHS IS0 19820:2016(E) Contents Page Foreword ..iv Introduction. ..V 1 Scope. ..1 2 Normative references ..1 3 Terms and definitions 4 Principle ..2 5 Test environment. ..2 6 Reagents, test organisms and media .2 6.1 Test organisms . 2 6.2 Culturing and dilution medium .2 6.3 Reference substance. .3 7 Apparatus .3 8 Treatment and preparation of samples .3 8.1 Special precautions .3 8.2 Preparation of the stock solutions of substances to be tested 4 9 Procedure. .4 9.1 Selection of test concentrations 4 9.2 Preparation of the test and control solutions .4 9.3 Introduction of the organisms 5 9.4 Incubation of the test system. .6 9.5 Measurements. .6 10 Estimation of the LC50 7 11 Reference test. ..7 12 Validity criteria ..7 13 Test report ..8 Annex A (informative) Procedure for hatching of Brachionus plicatilis cysts ..9 Annex B (informative) Preparation of artificial seawater ..10 Annex C (informative) Performance data ..11 Bibliography ..13 iii nsee=ZHEJIANG INST OF STANDARDIZATION C1 5956617 ithout license from IHS