ISO INTERNATIONAL STANDARD 19827 First edition 2016-02-15 Water quality Determination of the acute toxicity to the freshwater rotifer Brachionus calyciflorus Qualite de I'eau - Détermination de la toxicité aigue envers le rotifere d'eau douce Brachionus calyciflorus Reference number IS0 19827:2016(E) ISO International Organization for Standardization ZHEJIANG INST OF STANDARDIZATION C1 5956617 @IS02016 No reproduction or networking permitted without license from IHS IS0 19827:2016(E) COPYRIGHTPROTECTEDDOCUMENT IS0 2016, Published in Switzerland All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting on the internet or an intranet, without prior written permission. Permission can be requested from either ISO at the address below or ISO's member body in the country of the requester. ISO copyrightoffice Ch. de Blandonnet 8 · CP 401 CH-1214 Vernier, Geneva, Switzerland Tel. +41 22 749 01 11 Fax +41 22 749 09 47 copyright@iso.org www.iso.org Internatinai br DrganizationforStandardization Licensee-ZHEJIANG INST OF STANDARDIZAISQ 0d6 - All rights reserved HS und Not for Resale, 2016/4/26 02:27:27 networking permited without license from IHS IS0 19827:2016(E) Contents Page Foreword ..iv Introduction. ..V 1 Scope. ..1 2 Normative references ..1 3 Terms and definitions 4 Principle ..2 5 Test environment. .2 6 Reagents, test organisms and media 7 .3 Apparatus 8 Treatment and preparation of samples .3 8.1 Special precautions 8.2 Preparation of the stock solutions of substances to be tested 4 9 Procedure. .4 9.1 Selection of test concentrations ..4 9.2 Preparation of the test and control solutions .4 9.3 Introduction of the organisms ..5 9.4 Incubation of the test system .6 9.5 Measurements .6 10 Estimation of the LC50 ..7 11 Reference test .> 12 Validity criteria ..7 13 Test report Annex A (informative) Procedure for hatching of Brachionus calyciflorus cysts Annex B (informative) Preparation of artificial water ..10 Annex C (informative) Performance data ..11 Bibliography ..13 iii nSee=ZHEJIANG INST OF STANDARDIZATION C1 5956617 thout license from IHS