ISO INTERNATIONAL STANDARD 6579-1 First edition 2017-02 Microbiology of the food chain - Horizontal method for the detection, enumeration and serotyping of Salmonella - Part 1: Detection of Salmonella spp. Microbiologie de la chaine alimentaire - Meéthode horizontale pour la recherche, le dénombrement et le sérotypage des Salmonella- Partie 1: Recherche des Salmonella spp. Reference number ISO 6579-1:2017(E) rso International Organization for Standardization @ IS0 2017 HEJIANG INST OF STANDARDIZATION C1 5956617 ed without license from IHS IS0 6579-1:2017(E) COPYRIGHTPROTECTEDDOCUMENT IS0 2017, Published in Switzerland All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized otherwise in any form written permission. Permission can be requested from either ISO at the address below or ISO's member body in the country of the requester. ISOcopyrightoffice Ch. de Blandonnet 8 . CP 401 CH-1214 Vernier, Geneva, Switzerland Tel. +41 22 749 01 11 Fax +41 22 749 09 47 copyright@iso.org www.iso.org Internatinair PrganizationforStandardization ensee-ZHEJIANG INSTOF STANDARDIzARoISQa7 - All rights reserved Not for Resale, 2017/5/11 02:38:14 etworking permitted without license from IHS IS0 6579-1:2017(E) Contents Page Foreword ..V Introduction. ..vii 1 Scope. 2 Normative references 3 Terms and definitions 4 Principle .2 4.1 General .2 4.2 Pre-enrichment in non-selective liquid medium. .2 4.3 Enrichment in/on selective media. . 2 .2 4.4 Plating out on selective solid media 4.5 Confirmation .3 5 Culture media, reagents, and antisera ..3 6 Equipment and consumables ..3 7 Sampling ..4 8 Preparation of test sample ..4 9 Procedure (see diagrams in Annex A) ..4 9.1 Test portion and initial suspension ..4 9.2 Non-selective pre-enrichment. .4 9.3 Selective enrichment.. ..5 9.3.1 General. ..5 9.3.2 Procedure for food, animal feed samples, and environmental samples from the food production area. .5 9.3.3 Procedure for samples from the primary production stage ..5 9.4 Plating out. .6 9.4.1 General ..6 9.4.2 Procedure for food, animal feed samples, and environmental samples from the food production area. ..6 9.4.3 Procedure for samples from the primary production stage. .6 9.5 Confirmation .7 9.5.1 General 9.5.2 Selection of colonies for confirmation 1 9.5.3 Biochemical testing ..8 9.5.4 Serological testing. ..11 9.5.5 Interpretation of biochemical and serological reactions .11 9.5.6 Serotyping. ..12 10 Expression of results ..12 11 Performance characteristics ofthe method. .12 11.1 Interlaboratory studies. .12 11.2 Sensitivity ..12 11.3 Specificity ..12 11.4 LOD50 :12 12 Test report ..13 Annex A (normative) Diagrams of the procedures ..14 AnnexB(normative)Culturemediaandreagents ..17 Annex C (informative) Method validation studies and performance characteristics ..32 Annex D (normative) Detection of Salmonella enterica subspecies enterica serovars Typhi and Paratyphi .38 iii see=ZHEJIANG INST OF STANDARDIZATION C1 5956617 ed withoutlicense from IHS