ISO INTERNATIONAL STANDARD 9308-1 Third edition 2014-09-15 Water quality Enumeration of Escherichia coli and coliform bacteria Part 1: Membrane filtration method for waters with low bacterial background flora Qualité de I'eau-Dénombrement des Escherichia coli et des bactéries coliformes- Partie 1: Methode par filtration sur membrane pour les eaux a faible teneur en bactéries Reference number IS0 9308-1:2014(E) LSO @ IS0 2014 I without license from IHS Not for Resale IS0 9308-1:2014(E) COPYRIGHTPROTECTEDDOCUMENT ISO2014 All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting on the internet or an intranet, without prior written permission. Permission can be requested from either ISO at the address below or ISO's member body in the country of the requester. ISO copyright office Case postale 56:CH-1211 Geneva 20 Tel. + 41 22 749 01 11 Fax + 41 22 749 09 47 E-mail copyright@iso.org Web www.iso.org Published in Switzerland @ IS0 2014 - All rights reserved No reproductic networking permited without license from IHS Not for Resale IS0 9308-1:2014(E) Contents Page Foreword ..iv Introduction. ..V 1 Scope. ..1 2 Normative references ..1 3 Terms and definitions ..1 4 Principle ..2 5 Apparatus and glassware .2 6 Culture media and reagents ..2 7 Sampling. ..3 8 Procedure 8.1 Preparation of the sample ..3 8.2 Filtration. 3 8.3 Incubation and differentiation .3 9 Expression of results .4 10 Test report. ..4 11 Quality assurance ..4 11.1 General 4 11.2 Performance testing of Chromogenic Coliform Agar (CCA) 11.3 Performance testing of oxidase test. ..5 Annex A (informative) Further microbiological information on coliform bacteria ..6 1 Annex B (normative) Composition and preparation of culture media and reagents Annex C (informative) Performance characteristics .9 Bibliography .10 ii se with IS lout license from IHS Not for Resale