ISO INTERNATIONAL STANDARD 10705-4 First edition 2001-12-15 Water quality Detection and enumeration of bacteriophages - Part 4: Enumeration of bacteriophages infecting Bacteroides fragilis Qualite de I'eau Détection et dénombrement des bactériophages Partie 4: Denombrement des bacteriophages infectant Bacteroides fragilis Reference number ISO 10705-4:2001(E) @ ISO 2001 Copyright International Organization for Standardization No reproduction or networking permited without license from IHS Not for Resale ISO10705-4:2001(E) PDF disclaimer This PDF file may contain embedded typefaces. In accordance with Adobe's licensing policy, this file may be printed or viewed but shall not be edited unless the typefaces which are embedded are licensed to and installed on the computer performing the editing. In downloading this file, parties accept therein the responsibility of not infringing Adobe's licensing policy. The ISO Central Secretariat accepts no liability in this area. Adobe is a trademark of Adobe Systems Incorporated. Details of the software products used to create this PDF file can be found in the General Info relative to the file; the PDF-creation parameters were optimized for printing. Every care has been taken to ensure that the file is suitable for use by IsO member bodies. In the unlikely event that a problem relating to it is found, please inform the Central Secretariat at the address given below. @ISO2001 All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized in any form or by any means, member body in the country of the requester. ISO copyright office Case postale 56 · CH-1211 Geneva 20 Tel. + 4122 749 01 11 Fax + 41 22 749 09 47 E-mail [email protected] Web www.iso.ch Printed in Switzerland yatgzaiSandardizatn ISO 2001 - All rights reserved No reproduction or networking permitted without license from IHS Not for Resale ISO 10705-4:2001(E) Contents Page Scope 2 Normative references 3 Term and definitior 4 Safety precautions 5 Principle 2 6 Reagents 2 7 Apparatus 2 8 Microbiological reference cultures 9 Sampling ... 4 Preparation of test materials 11 Procedure 7 12 Expression of results 9 13 Test report 10 Annexes A General description of bacteriophages infecting Bacteroides fragilis 11 B Culture media and diluents ... 12 C Alternative method for preparing inoculum cultures. 17 D Culturing of Bacteriophage B56-3.. 18 Bibliography... 19 Copyright International Organization for Standardizationserved ii No reproduction or networking permited without license from IHS Not for Resale