ISO INTERNATIONAL STANDARD 19250 First edition 2010-07-15 Water quality Detection of Salmonella spp. Qualité de I'eau Recherche de Salmonella spp. Reference number ISO 19250:2010(E) @ ISO 2010 by IHS under lic itted without license from IHS Not for Resale ISO 19250:2010(E) PDF disclaimer This PDF file may contain embedded typefaces. In accordance with Adobe's licensing policy, this file may be printed or viewed but shall not be edited unless the typefaces which are embedded are licensed to and installed on the computer performing the editing. In downloading this file, parties accept therein the responsibility of not infringing Adobe's licensing policy. The IsO Central Secretariat accepts no liability in this area. Adobe is a trademark of Adobe Systems Incorporated. Details of the software products used to create this PDF file can be found in the General Info relative to the file; the PDF-creation parameters were optimized for printing. Every care has been taken to ensure that the file is suitable for use by IsO member bodies. In the unlikely event that a problem relating to it is found, please inform the Central Secretariat at the address given below COPYRIGHTPROTECTEDDOCUMENT ISO2010 All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized in any form or by any means, ISsO's member body in the country of the requester. ISO copyright office Case postale 56 . CH-1211 Geneva 20 Tel. + 41 22 749 01 11 Fax + 41 22 749 09 47 E-mail [email protected] Web www.iso.org Published in Switzerland @ ISO 2010 - All rights reserved py IHS unde I without license from IHS Not for Resale ISO 19250:2010(E) Contents Page Foreword Introductior 1 Scope 2 Normative references 3 Terms and definitions 4 Principle. 4.1 General 4.2 Pre-enrichment in non-selective liquid medium 4.3 Enrichmentinselective liquidmedia.... 4.4 Plating out and recognition... 4.5 Confirmation ... 5 Apparatus 6 Sampling. 7 Culture media and reagents . 8 Procedure... 8.1 Preparationofthesample 8.2 Non-selective pre-enrichment.... 8.3 Selective enrichment.. 8.4 Plating out . 8.5 Confirmation 9 Expression of results 10 Test report. 9 Annex A (normative) Diagram of procedure. 10 Annex B (normative) Composition and preparation of culture media and reagents.. 11 Annex C (informative) Results of the interlaboratory trial.. 18 Bibliography.... 23 CopyrightInternatinal Oorganizain for Standardizalion ghts reserved ii Not for Res: