ISO INTERNATIONAL STANDARD 22196 Second edition 2011-08-01 Measurement of antibacterial activity on plastics and other non-porous surfaces Mesurage de I'action antibacterienne sur les surfaces en plastique et autres surfaces non poreuses Reference number ISO 22196:2011(E) @ ISO 2011 yIHS under Not for Resale ISO 22196:2011(E) COPYRIGHTPROTECTEDDOCUMENT @ ISO2011 All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying and microfilm, without permission in writing from either isO at the address below or Iso's member body in the country of the requester. ISO copyright office Case postale 56 : CH-1211 Geneva 20 Tel. + 41 22 749 01 11 Fax + 41 22 749 09 47 E-mail [email protected] Web www.iso.org Published in Switzerland @ ISO 2011 - All rights reserved itted without license from IHS Not for Resale ISO 22196:2011(E) Contents Page Foreword .iv Introduction 1 Scope 2 Normative references. 3 Terms and definitions 4 Materials.. 4.1 Bacteria to be used for the tests. 4.2 Reagents, culture media and solutions 5 Apparatus 6 Sterilization of apparatus and storage of stock cultures. 6.1 Dry-heat sterilization .... 6.2 High-pressure steam sterilization.. 6.3 Preparation of glassware... 6.4 Maintenance of stock cultures. 7.1 Pre-culture of bacteria .... 7.2 Preparation of test specimens. 7.3 Preparation of test inoculum.. 7.4 Inoculation of test specimens. 7.5 Incubation of the inoculated test specimens .8 7.6 Recovery of bacteria from test specimens. 7.7 Determining the viable bacteria count by the pour plate culture method. 8 Expression of results... 8.1 Determination of the number of viable bacteria.... 8.2 Conditionsfora validtest.. 9 8.3 Calculation of the antibacterial activity.. 8.4 Effectiveness of the antibacterial agent.. 10 9 Repeatabilityandreproducibility 10 10 Test report.. 10 Annex A (normative) Quality of biological materials.. 11 A.1 General ..... A.2 Chemical composition of 1/500 nutrient broth (1/500 NB) .. 11 Annex B (informative) Repeatability and reproducibility.. B.1 Background.... 12 B.2 Summary 12 B.3 Experiment. 12 B.4 Results and discussion ... 13 Bibliography.. Copyrght International OrganizaionforStandardizalonghts reserved ii se from IHS Not for Resale