ISO INTERNATIONAL STANDARD 10130 First edition 2009-12-15 Cosmetics Analytical methods - Nitrosamines: Detection and determination of N-nitrosodiethanolamine (NDELA) in cosmetics by HPLC, post- column photolysis and derivatization Cosmetiques Methodes analytiques - Nitrosamines: Recherche et dosage de la N-nitrosodiethanolamine (NDELA) dans les cosmetiques par CLHP, photolyse et dérivation post-colonne Reference number ISO 10130:2009(E) @ ISO 2009 y IHS unde sefrom IHS Not for Resale ISO 10130:2009(E) PDFdisclaimer This PDF file may contain embedded typefaces. In accordance with Adobe's licensing policy, this file may be printed or viewed but shall not be edited unless the typefaces which are embedded are licensed to and installed on the computer performing the editing. In downloading this file, parties accept therein the responsibility of not infringing Adobe's licensing policy. The ISo Central Secretariat accepts no liability in this area. Adobe is a trademark of Adobe Systems Incorporated. Details of the software products used to create this PDF file can be found in the General Info relative to the file; the PDF-creation parameters were optimized for printing. Every care has been taken to ensure that the file is suitable for use by IsO member bodies. In the unlikely event that a problem relating to it is found, please inform the Central Secretariat at the address given below COPYRIGHTPROTECTED DOCUMENT @ISO2009 All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized in any form or by any means electronic or mechanical, including photocopying and microfilm, without permission in writing from either isO at the address below or Iso's member body in the country of the requester. ISO copyright office Case postale 56. CH-1211 Geneva 20 Tel. + 4122 749 01 11 Fax + 41 22 749 09 47 E-mail [email protected] Web www.iso.org Published in Switzerland @ISO2009-All rightsreserved I without license from IHS Not for Resale ISO 10130:2009(E) Contents Page Foreword Introduction 1 Scope 2 Principle. 3 Reagents 4 Apparatus. 5 Sample preparation and storage. 5.1 General. 5.2 Standards preparation 5.2.1 Primary stock solution.... 5.2.2 Secondary stock solution 5.2.3 Working solutions 5.3 Sample preparation. 5.3.1 5.3.2 SPE clean-up.. 5.3.3 Alternative sample preparation for samples non-dispersible in water (DcM clean-up) 5 6 Procedure 6.1 .5 6.2 Chromatographic conditions . 6.3 Set-up of the reaction system 7 Calculation of results .. 7.1 Calibration curve . 7.2 Experimentalconditionsforthevalidityofthemeasurement.. 7.3 Calculation of concentrations.... 8 Test report. Annex A (informative) Examples of calibration curves and typical chromatograms . Annex B (normative) Photolysis and reaction of nitrite with Griess reagent to form the azo dye.. Annex C (normative) Configuration of the post-column reactor system .12 Bibliography 13 Copyright International Organization for Standardizalon ghts reserved ii from IHS Not for Re