ISO INTERNATIONAL STANDARD 16000-17 First edition 2008-12-15 Indoor air - Part 17: Detection and enumeration of moulds Culture-based method Air intérieur- Partie 17: Detection et denombrement des moisissures - Methode par culture Reference number ISO 16000-17:2008(E) @ ISO 2008 IHSunder Not for Resale ISO 16000-17:2008(E) PDF disclaimer This PDF file may contain embedded typefaces. In accordance with Adobe's licensing policy, this file may be printed or viewed but shall not be edited unless the typefaces which are embedded are licensed to and installed on the computer performing the editing. In accepts no liability in this area. Adobe is a trademark of Adobe Systems Incorporated. Details of the software products used to create this PDF file can be found in the General Info relative to the file; the PDF-creation parameters were optimized for printing. Every care has been taken to ensure that the file is suitable for use by IsO member bodies. In the unlikely event that a problem relating to it is found, please inform the Central Secretariat at the address given below. COPYRIGHTPROTECTED DOCUMENT @ ISO2008 All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying and microfilm, without permission in writing from either isO at the address below or IsO's memberbody in the country of the requester. ISO copyright office Case postale 56. CH-1211Geneva 20 Tel. + 4122 749 01 11 Fax + 41 22 749 09 47 E-mail [email protected] Web www.iso.org Published in Switzerland @ ISo 2008 - All rights reserved I without license from IHS Not for Resale ISO 16000-17:2008(E) Contents Page Foreword Introduction vi 1 Scope 2 Normative reference 3 Definitions 4 Principle. 5 Apparatus 6 Culture media and diluents... 6.1 Dicloran 18 % glycerol agar (DG18 agar) 6.2 Malt-extract agar .. 6.3 Potato dextrose agar 6.4 Saline solution. 6.5 Saline solution with polysorbate 80. 7 Procedure 7.1 General. 7.2 Processing of filters 7.3 Incubation.. 7.4 Examination and counting. 7.5 Identification of mould species 7.6 Calculationandexpressionofresults.. 8 Test report... 11 Annex A (informative) Specific characteristics of mould spores. 12 Annex B (informative) Exchange of samples for validation of the cultivation method Bibliography ii from IHS Not for Resi