ISO INTERNATIONAL STANDARD 22303 First edition 2008-07-15 Tobacco Determination of tobacco specific nitrosamines Method using buffer extraction Tabac Dosage des nitrosamines spécifiques du tabac - Méthode d'extraction par solution tampon Reference number ISO 22303:2008(E) @ ISO 2008 by IHS under lic Not for Resale ISO 22303:2008(E) PDF disclaimer This PDF file may contain embedded typefaces. In accordance with Adobe's licensing policy, this file may be printed or viewed but shall not be edited unless the typefaces which are embedded are licensed to and installed on the computer performing the editing. In downloading this file, parties accept therein the responsibility of not infringing Adobe's licensing policy. The IsO Central Secretariat accepts no liability in this area. Adobe is a trademark of Adobe Systems Incorporated. Details of the software products used to create this PDF file can be found in the General Info relative to the file; the PDF-creation parameters were optimized for printing. Every care has been taken to ensure that the file is suitable for use by IsO member bodies. In the unlikely event that a problem relating to it is found, please inform the Central Secretariat at the address given below. COPYRIGHT PROTECTED DOCUMENT @ ISO2008 All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized in any form or by any means, IsO's member body in the country of the requester. ISO copyright office Case postale 56 . CH-1211 Geneva 20 Tel. + 41 22 749 01 11 Fax + 41 22 749 09 47 E-mail [email protected] Web www.iso.org Published in Switzerland @ IS0 2008 - All rights reserved py IHS unde I without license from IHS Not for Resale ISO 22303:2008(E) Contents Page Foreword iv Introduction 1 Scope 2 Normative references 3 Terms and definitions 4 Principle. 5 Reagents 6 Apparatus.. Preparation of extraction solution and standards 7.1 Preparation of buffer extraction solution .. 7.2 Preparation of internal standard ... 7.3 Preparation of calibration standards .... 7.4 Setting up of the gas chromatograph and detector 7.5 Tobacco extraction and isolation of TSNAs .. 7.6 Extractconcentration 8 Data analysis and calculation of results , 9 Repeatability and reproducibility 10 Test report Annex A (informative) Typical chromatograms and calibration curves .. Bibliography... Copyrght International Organization for standardizalon ghts reserved ii nse from IHS Not for Resale