1041 NTERNATIONAL STANDARD (ISO) INTERNATIONAL ORGANIZATION FOR STANDARDIZATION -MEKAYHAPOAHA OPIAHW3ALIM IO CTAHJIAPTW3ALIWM·ORGANISATION INTERNATIONALE DE NORMALISATION Essential oils - Determination of freezing point First edition - 1973-12-01 NOV 1 21973 Mvs Interlcat Doa Sect SO1041-1973(E) UDC668.5:536.421.4 Ref.No.ISO1041-1973(E) Descriptors:Essential oils,tests,physical tests,solidificationpoints. Price based on 2 pages r Standardizatior No reproduction or networking permitted without license from IHS Not for Resale FOREWORD Iso (the International Organization for Standardization) is a woridwide federation of national standards institutes (isO Member Bodies). The work of developing International Standards is carried out through Iso Technical Committees. Every Member Body interested in a subject for which a Technical Committee has been set up has the right to be represented on that Committee. International organizations, governmental and non-governmentai, in liaison with iso, also take part in the work. Draft International Standards adopted by the Technicai Committees are circulated to the Member Bodies for approval before their acceptance as International Standardsbythe isO Council. Prior to 1972, the results of the work of the Technical Committees were published as Iso Recommendations; these documents are now in the process of being transformed into International Standards. As part of this process, Technical Committee ISO/TC 54 has reviewed ISO Recommendation R1041 and found it suitable for transformation. International Standard ISo therefore replaces IsO Recommendation R 1041-1969. IS0Recommendation R1041 was approved by the Member Bodies of the following countries : Argentina Germany Portugal Australia Greece Romania Austria India Spain Belgium Italy Sweden Canada Morocco United Kingdom Chile Netherlands U.S.S.R. France NewZealand Yugoslavia No Member Body expressed disapproval of the Recommendation. @ Internationat Organization for Standardization,1973 Printed in Switzerland Copyright Internatinal Organizationfor Standardization Noreproductionornetworkingpemitedwithout licensefromHS Not for Resale ISO 1041-1973 (E) INTERNATIONAL STANDARD Essential oils -- Determination of freezing point 1 SCOPEANDFIELDOFAPPLICATION 5.4 Apparatus assembly for determination of freezing point, consisting of a wide-mouthed container of about This International Standard specifies a method 500 ml capacity, provided with a bored cork or rubber determining the freezing points of essential oils. It is not stopper into which the stout-walled test tube (5.3) is applicable in the special case of essential oil of rose. inserted. The test tube (5.2) is fitted into the stout-walled test tube (5.3) by means of another bored cork or rubber 2 REFERENCES stopper.Into the test tube (5.2)a thermometer(5.1)is inserted, so that the centre of its mercury bulb is located at ISo 212, Essential oils -- Sampling. the centre of the liquid. iSO/R356,Essentialoils-Methods of test-Preparation A suitable apparatus assembly is shown in the figure. ofsample. 3 DEFINITION 6 SAMPLING For the purpose of this International Standard,the See ISO 212. following definition applies : freezing point: Either the constant temperature, or the maximum temperature, observed when the oil, in 7 PROCEDURE supercooled liquid state, liberates its latent heat of fusion. 7.1 Preparation of test sample 4 PRINCIPLE See ISO/R 356. Slow and progressive cooling of the essential oil. 7.2 Preliminary test Observation of the variations in temperature as the oil passes from the liquid to the solid state. If necessary, first liquefy the essential oil by warming. Coo a few millilitres of the oil to be tested in a small test tube, and stir with a thermometer until solidification takes place. 5APPARATUS Note the temperature and set aside in a cooi place. 5.1 Calibrated thermometers, fuffilting the following requirements: 7.3 Determination length of bulb : 10 to 20 mm Fill the container (see 5.4) wit